Purification of DNA from formaldehyde fixed and paraffin embedded human tissue

The ability to isolate DNA from preserved human tissues would provide numerous experimental opportunities. In this report it is shown that DNA can be extracted from tissues prepared for routine histopathological examination (i.e., fixed with formaldehyde and embedded in paraffin). Although the extra...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 130; no. 1; pp. 118 - 126
Main Authors Goelz, SE, Hamilton, SR, Vogelstein, B
Format Journal Article
LanguageEnglish
Published 01.01.1986
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Summary:The ability to isolate DNA from preserved human tissues would provide numerous experimental opportunities. In this report it is shown that DNA can be extracted from tissues prepared for routine histopathological examination (i.e., fixed with formaldehyde and embedded in paraffin). Although the extracted DNA is not intact, it is double stranded, cleavable with restriction endonucleases, and suitable for a variety of standard techniques used in molecular biology.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0006-291X