Toxigenic Fusarium spp. as Determinants of Thichothecene Mycotoxins in Settled Grain Dust

Toxigenic Fusarium spp. as determinants of trichothecene mycotoxins in settled grain dust were presented. Mycotoxins were determined by gas chromatography-mass spectrometry, whereas the Fusarium spp. were identified and quantified both by species-specific semiquantitative polymerase chain reaction (...

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Published inJournal of occupational and environmental hygiene Vol. 3; no. 12; p. 651
Main Authors Halstensen, Anne Straumfors, Nordby, Karl-Christian, Klemsdal, Sonja Sletner, Elen, Oleif, Clasen, Per-Erik, Eduard, Wijnand
Format Journal Article
LanguageEnglish
Published 01.12.2006
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Summary:Toxigenic Fusarium spp. as determinants of trichothecene mycotoxins in settled grain dust were presented. Mycotoxins were determined by gas chromatography-mass spectrometry, whereas the Fusarium spp. were identified and quantified both by species-specific semiquantitative polymerase chain reaction (PCR) and by cultivation. It was observed that all potential trichothecene-producing molds in the grain dust were quantified using a PCR assay specific for tri5, the gene coding for trichothecene biosynthesis. It was concluded that PCR clearly improved the detection of toxigenic Fusaria as potential sources of health risks for farmers inhaling grain dust work, but the use of Fusarium-DNA as indicators for trichothecenes should be used cautiously.
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ISSN:1545-9624