How lambda repressor and lambda Cro distinguish between O sub(R)1 and O sub(R)3

Although lambda repressor and lambda Cro bind to the same six operators on the phage chromosome, the fine specificities of the two proteins differ: repressor binds more tightly to O sub(R)1 than to O sub(R)3, and vice versa for Cro. In this paper, the authors change base pairs in the operators and a...

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Bibliographic Details
Published inCell Vol. 47; no. 5; pp. 807 - 816
Main Authors Hochschild, A, Douhan, J III, Ptashne, M
Format Journal Article
LanguageEnglish
Published 01.01.1986
Subjects
phage lambda
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Summary:Although lambda repressor and lambda Cro bind to the same six operators on the phage chromosome, the fine specificities of the two proteins differ: repressor binds more tightly to O sub(R)1 than to O sub(R)3, and vice versa for Cro. In this paper, the authors change base pairs in the operators and amino acids in the proteins to analyze the basis for these differences. The results show how repressor and Cro recognize and distinguish between two related operator sequences.
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ISSN:0092-8674