Influence of Cl super(-) on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver

• Published in: The Journal of clinical investigation Vol. 79; no. 4; pp. 1259 - 1268
• Main Authors: Wolkoff, A W, Samuelson, A C, Johansen, K L, Nakata, R, Withers, D M, Sosiak, A
• Format: Journal Article
• Language: English
• Published: 01.01.1987
• Subjects: anions; chloride; hepatocytes; liver
• ISSN: 0021-9738

Transport of super(35)S-labeled sulfobromophthalein ( super(35)S)BSP was studied in short-term cultured rat hepatocytes incubated in bovine serum albumin. At 37 degree C, initial uptake of ( super(35)S)BSP was 5-10-fold that at 4 degree C, linear for at least 15 min, saturable, inhibited by bilirubin, and reduced by > 70% after ATP depletion or isosmotic substitution of sucrose for NaCl in medium. Transport of ( super(3)H)bilirubin was studied in isolated livers perfused with control medium or medium in which Cl super(-) was replaced by gluconate super(1). Perfusion data, revealed 40-50% reductions in influx and efflux with gluconate super(-) substitution. The results are consistent with existence of a Cl super(-)/organic anion-exchange mechanism similar to that described by others in renal tubules.