Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field super(17)O MR spectroscopy

To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. super(17)O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMR...

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Published inJournal of cerebral blood flow and metabolism Vol. 33; no. 12; pp. 1846 - 1849
Main Authors Cui, Weina, Zhu, Xiao-Hong, Vollmers, Manda L, Colonna, Emily T, Adriany, Gregor, Tramm, Brandon, Dubinsky, Janet M, Oez, Guelin
Format Journal Article
LanguageEnglish
Published 01.12.2013
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Summary:To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. super(17)O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO sub(2)) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO sub(2) measurements with super(17)O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.
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ISSN:0271-678X
DOI:10.1038/jcbfm.2013.172