Induced gynogenesis by retention of the second polar body in the common carp, Cyprinus carpio L., and heterozygosity of gynogenetic progeny in transferrin and Ldh-B super(1) loci

• Published in: Aquaculture Vol. 54; no. 1-2; pp. 63 - 67
• Main Authors: Linhart, O, Kvasnicka, P, Slechtova, V, Pokorny, J
• Format: Journal Article
• Language: English
• Published: 01.01.1986
• Subjects: aquaculture; aquaculture techniques; biotechnology; Cyprinus carpio; fish culture; Freshwater; genomes; gynogenesis; induced breeding; polyploids; selective breeding
• ISSN: 0044-8486

For two years (1983 and 1984), experiments on induced gynogenesis (egg development triggered by genetically inactivated sperm) by the retention and fusion of the second polar body were carried out in the common carp (Cyprinus carpio ). Gamma rays ( super(60)Co, absorbed dose 1000 Gy) were used to inactivate the spermatozoan DNA. To restore the diploid constitution, eggs were cold-shocked (at 0-4 degree C for 60 min) at 5 and 15 min after activation of spermatozoa and eggs by the fertilization solution. The gynogenetic nature of the progeny (i.e., the absence of the paternal genome) was tested for various loci by checking on scaliness, transferrin and Ldh-B super(1) phenotypes. In the mirror genotype, great variability was observed in the amount and localization of scales. The progeny heterozygosities in the transferrin locus were 0.95% (of 1 female) and 5.56% (of 4 females) in 1983 and 1984, respectively. The progeny heterozygosities in the Ldh-B super(1) locus were 38.84% (of 2 females) and 42.42% (of 2 females) in 1983 and 1984, respectively.