Inositol phosphate formation in fMet-Leu-Phe-stimulated human neutrophils does not require an increase in the cytosolic free Ca super(2+) concentration

The accumulation of inositol phosphates in myo-( super(3)H)inositol-labelled human neutrophils stimulated with the chemotactic peptide fMet-Leu-Phe was measured. The challenge with the chemotactic peptide caused the generation of inositol monophosphate (InsP), inositol bisphosphate (InsP sub(2)) and...

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Published inBiochemical journal Vol. 229; no. 2; pp. 361 - 367
Main Authors Di Virgilio, F, Vicentini, L M, Treves, S, Riz, G, Pozzan, T
Format Journal Article
LanguageEnglish
Published 01.01.1985
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Summary:The accumulation of inositol phosphates in myo-( super(3)H)inositol-labelled human neutrophils stimulated with the chemotactic peptide fMet-Leu-Phe was measured. The challenge with the chemotactic peptide caused the generation of inositol monophosphate (InsP), inositol bisphosphate (InsP sub(2)) and inositol trisphosphate (InsP sub(3)). The formation of the three inositol phosphates followed a differential time course: InsP sub(3) accumulated very rapidly and transiently, whereas InsP increased steadily for more than 2 min. Inositol phosphate formation was only partially decreased by procedures which prevented the fMet-Leu-Phe-dependent increase of cytosolic free Ca super(2+) concentration.
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ISSN:0264-6021