Further characterization of cardiodigin, Na super(+), K super(+)-ATPase inhibitor extracted from mammalian tissues

This study was undertaken to characterize endogenous digitalis-like activity in water extract from mammalian tissues. Prepurified samples obtained from guinea-pig heart were analysed by reverse-phase HPLC using an acetonitrile gradient. The eluent was assayed for its activity as inhibitor of human h...

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Bibliographic Details
Published inFEBS letters Vol. 184; no. 1; pp. 150 - 154
Main Authors Fagoo, M, Godfraind, T
Format Journal Article
LanguageEnglish
Published 01.01.1985
Subjects
adenosinetriphosphatase
cardiodigin
digoxin
guinea-pigs
heart
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Summary:This study was undertaken to characterize endogenous digitalis-like activity in water extract from mammalian tissues. Prepurified samples obtained from guinea-pig heart were analysed by reverse-phase HPLC using an acetonitrile gradient. The eluent was assayed for its activity as inhibitor of human heart Na super(+),K super(+)-ATPase and digoxin-like immunoreactivity. Both activities were recovered in the same fraction after two successive chromatographic steps. These results provide further evidence for the presence of an endogenous digitalis like factor, cardiodigin, in mammalian heart.
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ISSN:0014-5793