Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor

A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the delta subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector lambda gt10 from membrane associated poly(A) super(+) RNA from BC3H-1 mouse...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 81; no. 24; pp. 7971 - 7974
Main Authors LaPolla, R J, Mayne, K M, Davidson, N
Format Journal Article
LanguageEnglish
Published 01.01.1984
Subjects
Torpedo
Online AccessGet full text

Cover

More Information
Summary:A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the delta subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector lambda gt10 from membrane associated poly(A) super(+) RNA from BC3H-1 mouse cells. Surprisingly, the delta clone was selected by hybridization with cDNA encoding the gamma subunit of the Torpedo AcChoR. The mouse protein has the same general structural features as do the )Torpedosubunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
content type line 23
ObjectType-Feature-1
ISSN:0027-8424