Discrimination among the human beta super(A), beta super(S), and beta super(C)-globin genes using allele-specific oligonucleotide hybridization probes

Synthetic nonadecanucleotides complementary to the human beta super(A)-, beta super(S)-, or beta super(C)-globin sequences were used as hybridization probes to screen human genomic DNA samples for these genes. The oligonucleotides were super(32)P-labeled and used as probes to genotype restriction en...

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Bibliographic Details
Published inAmerican journal of human genetics Vol. 37; no. 1; pp. 42 - 51
Main Authors Studencki, AB, Conner, B J, Impraim, C C, Teplitz, R, Wallace, R B
Format Journal Article
LanguageEnglish
Published 01.01.1985
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Summary:Synthetic nonadecanucleotides complementary to the human beta super(A)-, beta super(S)-, or beta super(C)-globin sequences were used as hybridization probes to screen human genomic DNA samples for these genes. The oligonucleotides were super(32)P-labeled and used as probes to genotype restriction endonuclease digests of human genomic DNA. The data obtained show that hybridization with oligonucleotide probes, unlike restriction fragment length polymorphism analysis or direct restriction enzyme digestion, can be used to directly distinguish among the three alleles of beta -globin, beta super(A), beta super(S), and beta super(C), when present either in one (heterozygous) or two copies.
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SourceType-Scholarly Journals-1
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ISSN:0002-9297