Effects of diethyldithiocarbamate and nine other nucleophiles on the intersubunit protein cross-linking and inactivation of purified human alpha sub(2)-macroglobulin by cis-diamminedichloroplatinum(II)

The cross-linking and inactivation of the plasma protein alpha sub(2)-macroglobulin by cis-diamminedichloroplatinum(II) (cisplatin) was used to study platinum(II)-protein binding in the presence of compounds of therapeutic or biochemical significance. Diethyldithiocarbamate, potassium cyanide (KCN),...

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Published inCancer research (Chicago, Ill.) Vol. 44; no. 12; pp. 5764 - 5770
Main Authors Gonias, S L, Oakley, A C, Walther, P J, Pizzo, S V
Format Journal Article
LanguageEnglish
Published 01.01.1984
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Summary:The cross-linking and inactivation of the plasma protein alpha sub(2)-macroglobulin by cis-diamminedichloroplatinum(II) (cisplatin) was used to study platinum(II)-protein binding in the presence of compounds of therapeutic or biochemical significance. Diethyldithiocarbamate, potassium cyanide (KCN), sodium thiocyanate, L-methionine, N-acetyl-L-cysteine, 2-amino-ethanethiol, L-cysteine, L-lysine, L-histidine, and L-arginine demonstrated variable capacity to inhibit reaction of cisplatin with protein and to reverse bidentate platinum(II)-protein binding in the in vitro model system. alpha sub(2)-Macroglobulin lost 90% of its activity and was completely cross-linked, as determined with polyacrylamide gel electrophoresis, after reaction with cisplatin (0.6 to 1.0 mM). When diethyldithiocarbamate (4 to 15 mM) was incubated with alpha sub(2)-macroglobulin and cisplatin, protein inactivation and cross-linking were totally prevented.
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ISSN:0008-5472