Creatine kinase in regulation of heart function and metabolism. II. The effect of phosphocreatine on the rigor tension of EGTA-treated rat myocardial fibers

Bundles of rat cardiac fibers were treated with EGTA to increase the permeability of the sarcolemma to ions and small molecules. In the medium without calcium, the EGTA-treated fibers developed rigor tension dependent on the concentration of MgATP in the bathing solution. The results demonstrate tha...

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Published inBiochimica et biophysica acta, Protein structure and molecular enzymology Vol. 803; no. 4; pp. 265 - 270
Main Authors Veksler, VI, Kapelko, VI
Format Journal Article
LanguageEnglish
Published 01.01.1984
Subjects
cardiac muscle
creatine kinase
membrane permeability
rats
sarcolemma
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Summary:Bundles of rat cardiac fibers were treated with EGTA to increase the permeability of the sarcolemma to ions and small molecules. In the medium without calcium, the EGTA-treated fibers developed rigor tension dependent on the concentration of MgATP in the bathing solution. The results demonstrate that the intact creatine kinase in the EGTA-treated fibers with increased sarcolemmal permeability is able to ensure rapid replenishment of MgATP in the myofibrillar compartment at the expense of phosphocreatine. The data obtained conform completely to the concept of adenine-nucleotide compartmentation in cardiac cells and of energy channelling by the phosphocreatine-creatine shuttle mechanism.
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ISSN:0167-4838