The effect of essential fatty acid deficiency on the stimulation of intestinal calcium transport by 1,25-dihydroxyvitamin D sub(3)

• Published in: The Journal of biological chemistry Vol. 258; no. 8; pp. 4977 - 4981
• Main Authors: Kreutter, D, Matsumoto, T, Peckham, R, Zawalich, K, Wen, W H, Zolock, D T, Rasmussen, H
• Format: Journal Article
• Language: English
• Published: 01.01.1983
• ISSN: 0021-9258

The effect of altering the lipid composition of the brush-border membrane on the ability of 1,25-dihydroxyvitamin D sub(3) (1,25-(OH) sub(2)D sub(3)) to stimulate calcium transport across the intestinal mucosa was examined by raising chicks on a vitamin D, essential fatty acid deficient diet (-DEFAD) and measuring calcium absorption from duodenal sacs in situ and calcium uptake into brush-border membrane vesicles in vitro. Administration of 1,25-(OH) sub(2)D sub(3) to - DEFAD and to - D control chicks led to the same calcium transport in isolated brush -border membrane vesicles was not in the control group. The results support the concept that the action of 1,25-(OH) sub(2)D sub(3) on membrane lipid turnover and structure plays a critically important role in the 1,25-(OH) sub(2)D sub(3)-mediated cellular transport responses.