Hormonal influence on the in vitro bud burst of some cassava varieties and accessions from Benin

• Published in: African journal of biotechnology Vol. 12; no. 13; pp. 1475 - 1481
• Main Authors: Cacai, G, Ahanhanzo, C, Adjanohoun, A, Houedjissin, S, Azokpota, P, Agbangla, C
• Format: Journal Article
• Language: English
• Published: 27.03.2013
• Subjects: Manihot esculenta
• ISSN: 1684-5315; 1684-5315
• DOI: 10.5897/AJB12.2800

This work tested the effect of different growth regulators on Manihot esculenta explants cultured. Nodal segments were disinfected and cultivated on Murashige and Skoog's basal media. The effects of the different combinations on bud burst were observed after five weeks of culture. The results show an interaction between the growth regulators and the genotypes of the varieties and accessions. Most of the varieties and accessions (Gbeze, Kpetevikoutou, Ibadan and Sekandji) obtained the maximal bud burst in the media MS supplemented with 0.2 mg/l BAP and MS supplemented with 0.2 mg/l KIN. However, with segments cultivated in medium, containing 0.2 mg/l KIN, 1 in 15 showed bud burst for the accession Agric Come. Naphthalene acetic acid effect varied according to the genotype and the cytokinin used, whereas on 0.1 mg/l NAA combined with 0.2 mg/l with the variety 92/0057, 14 in 15 budded. However, no bud burst was observed with 0.1 mg/l NAA combined with 0.2 mg/l BAP.