Transdifferentiation of The Adipose Tissue-Derived Stem Cells into Neuron-Like Cells Ex pressing Neurotrophins by Selegiline

• Published in: Cell journal (Yakhteh) Vol. 14; pp. 25 - 26
• Main Authors: Abdanipour, A, Tiraihi, T, Delshad, A R
• Format: Journal Article
• Language: English
• Published: 01.01.2012
• Subjects: Adipose tissue; Brain-derived neurotrophic factor; CD45 antigen; CD49d antigen; CD90 antigen; Differentiation; Immunoreactivity; Nestin; Neurodegenerative diseases; Neurofilaments; Neurotrophins; Selegiline; Stem cells; Synapsin; Unloading
• ISSN: 2228-5806

Objective: Adult stem cells (ASC) are undifferentiated cells found throughout the body. These cells are promising tools for cell replacement therapy in neurodegenerative disease. Adipose tissue is the most abundant and accessible source of ASC. This study was conducted to evaluate effect of selegiline on differentiation of adipose-derived stem cells (ADSC) into functional neuronlike cells (NLC), and also level of the neurotrophin expression in differentiated cells. Materials and Methods: ADSC were transdifferentiated into NLC using selegiline where CD90, CD49d, CD31, CD 106 and CD45 were used as markers for ADSC identification. Lipogenic and osteogenic differentiation of ADSC were used to characterize the ADSC. ADSC were treated with selegiline at different concentrations (from 10 super(-6)to 10 super(-11) mM) and time points (3, 6, 12, 24 and 48 hours). Percentage of viable cells, nestin and neurofilament 68 (NF-68) immunoreactive cells were used as markers for differentiation. The optimal dose for neurotrophin expressions in differentiating cells was evaluated using reverse transcriptase-PCR. NLC function was evaluated by loading and unloading with FM1-43 dye. Results: ADSC were immunoreactive to CD90 (95.67 plus or minus 2.26), CD49d (71.52 plus or minus 6.64) and CD31 (0.6 plus or minus 0.86), but no immunoreactivity was detected for CD 106 and CD45. The results of neural differentiation showed the highest percentage of nestin and NF-68 positive cells at 10-9 mM concentration of selegiline (exposed for 24 hours). The differentiated cells expressed synapsin and neurotrophin genes except brain-derived neurotrophic factor. Conclusion: ADSC can be an alternative source in cell-based therapy for neurodegenerative diseases using selegiline to induce ADSC differentiation to neuronal lineage.