Monitoring of toxic microalga Ostropsis (Dinoflagellate) species in Mediterranean coastal waters using the PCR based-assay combined with light microscopy

A molecular PCR based-assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages in order to detect toxic Ostreopsis species in the Mediterranean Sea. The PCR allowed rapid detection of Ostreopsis cells, even if their abundan...

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Published inBiologia marina mediterranea Vol. 17; no. 1; pp. 362 - 363
Main Authors Battocchi, C, Totti, C, Vila, M, Maso, M, Capellacci, S, Accoroni, S, Rene, A, Scardi, M, Penna, A
Format Journal Article
LanguageEnglish
Published 01.03.2010
Subjects
Marine
Ostreopsis
MED, Central Mediterranean
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Summary:A molecular PCR based-assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages in order to detect toxic Ostreopsis species in the Mediterranean Sea. The PCR allowed rapid detection of Ostreopsis cells, even if their abundances are below the light microscopy's detection limit. Species-specific identification was possible only by PCR-based assay, due to the inherent difficulty of morphological identification in field samples. During the monitoring of the toxic Ostreopsis blooms PCR based methods proved to be effective tools complementary to microscopy for rapid and specific detection of Ostreopsis in marine coastal waters.
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ISSN:1123-4245