Study of Gene Expression of TrkA, TrkB, TrkC Tyrosine Kinase Receptors and P75 Receptor in Different Steps of Human Dental Pulp Stem Cells Differentiation

• Published in: Cell journal (Yakhteh) Vol. 14; p. 37
• Main Authors: Esmaeili, A, Pourveiseh, A, Nourbakhsh, N, Talebi, A, Esfahani, NMH
• Format: Journal Article
• Language: English
• Published: 01.01.2012
• Subjects: Alzheimer's disease; Cell survival; Central nervous system; Dental pulp; Differentiation; Gene expression; Ischemia; Multiple sclerosis; Neurodegenerative diseases; Neurotrophin receptors; Polymerase chain reaction; Primers; Protein-tyrosine kinase receptors; RNA; Signal transduction; Spinal cord injury; Stem cells; TrkA receptors; TrkB receptors
• ISSN: 2228-5806

Objective: Dental pulp stem cells are cells which can be used in treatment of neuro-degenerative diseases and injuries to the central nervous system including brain ischemia and spinal cord trauma. These cells produce variety of neurotrophic factors which are bind to TrkA, TrkB, TrkC tyrosine kinase receptors. Each neurotrophin has its special tyrosine kinase receptor, while P75 receptor is a common receptor and is able to bind to all of these factors. These receptors with their interaction with neurotrophins have roles in survival, differentiation and cell death due to stimulation of signaling pathways. In this study, we investigated the expression levels of these factors in different steps of dental pulp stem cell differentiation. Given that the increase of these receptors is as a result of increasing in neurotrophin's expression and they are involved in some neurodegenerative diseases such as, multiple sclerosis (MS) and Alzheimer's, we can use these receptors in treatment of these diseases. Materials and Methods: We designed specific primers for each neurotrophin receptor genes. We extracted cellular RNA content from stem cells in different steps of differentiation using RNeasy mini kit and then we make their cDNA. Finally, we can evaluated changes in gene expression using Real time PCR machine. Results: Results of this study showed that these receptors express in different levels in dental pulp stem cells. Conclusion: So, it can be concluded that they are important in differentiation of these stem cells and we can use them in treatment of neurodegenerative diseases.