Study of Gene Expression of BDNF, NGF, NT3 and NT4 Neurotrophins in Different Stagesof Human Dental Pulp Stem Cells Differentiation

• Published in: Cell journal (Yakhteh) Vol. 14; pp. 36 - 37
• Main Authors: Esmaeili, A, Alifarja, S, Nourbakhsh, N, Talebi, A, Esfahani, NMH
• Format: Journal Article
• Language: English
• Published: 01.01.2012
• Subjects: Alzheimer's disease; Brain-derived neurotrophic factor; Cell survival; Computer programs; Dental pulp; Differentiation; Gene expression; Growth factors; Movement disorders; Nerve growth factor; Neurodegenerative diseases; Neurotrophin 3; Neurotrophin 4; Parkinson's disease; Polymerase chain reaction; Primers; software; Stem cells
• ISSN: 2228-5806

Objective: Neurotrophins are growth factors which have roles in development of neural cells. Dental pulp stem cells are one of cell types that produce neurotrophins. These factors strongly affect on division, differentiation, survival and death of neural cells. NT4, NT3, NGF and BDNF neurotrophins are produced in dental pulp stem cells: lead these cells into neural cells. Studies have been demonstrated that, level of these factors, increase in inducing dental pulp stem cells. Given the limited nature of stem cells regions and easy and reliable availability to them, we can use these cells as a suitable source of stem cells. These cells can be used in clinical and therapeutic fields, especially in neurologic and neural disorders such as Alzheimer's, Huntington and Parkinson diseases. In this study, the mRNA expression level of neurotrophins have been studied in different stages of dental pulp stem cells differentiation. Materials and Methods: First, we designed specific primers of neurotrophins using Oligo and Beacon Designer software's. Then we used RNeasy mini kit in different stages of dental pulp stem cells differentiation to isolate cellular RNA and then cDNA was synthesized. After that, using Real time PCR. quantitative comparison of expression level of these genes was done. Results: Results of these experiments show that neurotrophins are expressed in different stages of differentiation. Conclusion: We can conclude that these factors are effective in cell differentiation and are useful in research and therapeutic goals.