The Expression of CD44 in Cord Blood CD34+ Cells Affected by Substance P

• Published in: Cell journal (Yakhteh) Vol. 13; p. 47
• Main Authors: Shahrokhi, S, Alimoghaddam, K, Ebtekar, M
• Format: Journal Article
• Language: English
• Published: 01.01.2011
• Subjects: CD34 antigen; CD44 antigen; Cell adhesion molecules; Cell culture; Cell migration; Cord blood; Cytokines; Interleukin 3; Interleukin 6; Liquid culture; Microenvironments; Neurokinin receptors; Neuropeptides; Stem cells; Substance P; Transplantation
• ISSN: 2228-5806

Objective: Modulating the expression of cell adhesion molecules on hematopoietic stem cells (HSCs) of cord blood (CB) may help to improve homing process of CB stem cells. Microenvironment and released mediators are major factors in the process of engraftment. Based on evidence concerning the existence of substance P (SP) receptor on cord blood CD34+ cells, we aimed to explore effects of SP on CD44 expression on CB-HSC. Materials and Methods: CD34+cells purified from CB, were cultured in a serum-free liquid culture system. Different concentrations of SP were used in combination with cytokine cocktail of SCF, FL, TPO, IL3 and IL6. Control groups were treated with cytokine cocktail. CD44 expression was enumerated by flowcytometry. Results: Our results show significant increase in frequency of SP treated cells at 10-7M and 10-11 M following 7 days cultivation as compared to control group. Additionally median flowcytometric intensity of CD44 at corresponding culture period and SP concentrations were significantly increased compare to freshly purified CD34+cells at day 0. Conclusion: Our findings suggest that SP neuropeptides could act as a novel supplement for cytokine cocktails to maintain or increased CD44 expression which could be beneficial in homing of cells in transplantation.