PPAR gamma Influences on Neural Precursor Cells Formation and Neural Differentiation of Mouse Embryonic Stem Cells

• Published in: Cell journal (Yakhteh) Vol. 13; pp. 32 - 33
• Main Authors: Ghaedi, K, Ghoochani, A, Shabani, K, Peymani, M, Karamali, F, Karbalaei, K, Tanhaei, S, Salamian, A, Esfahani, NMH, Baharvand, H
• Format: Journal Article
• Language: English
• Published: 01.01.2011
• Subjects: Astrocytes; Data processing; Differentiation; Embryo cells; Homeostasis; Inflammation; Insulin; Lipid metabolism; Neural stem cells; Neurogenesis; Nuclear receptors; Peroxisome proliferator-activated receptors; Polymerase chain reaction; rosiglitazone; Stem cells; Transcription factors
• ISSN: 2228-5806

Objective: Peroxisome proliferator activated receptors (PPARs), consisting three major isoforms termed alpha , beta / delta and gamma , are ligand-dependent transcription factors which are classified as one of the members of nuclear hormone receptors super family. Among them, PPAR gamma is involved in different metabolic activities including lipid metabolism, anti-inflammatory responses and regulation of tissue homeostasis, insulin sensitivity, development and differentiation. To address the importance of PPAR gamma on neural differentiation of mouse embryonic stem cells, the present study was designed and carried out during two different stages in process of neurogenesis by application of PPAR gamma selective agonist and antagonist. Materials and Methods: Royan B1 cell lines derived from the C57BL/6 strain were treated with either Rosiglitazone or GW9662, during or post neural precursor cell formation. Different approaches as Real Time PCR, immunostaining were implemented to chase the fate of neural cells during differentiation. Results: Data indicated that a decrease in the expression level of mature astrocyte marker post neural precursor cell formation treatment by inactivation of PPAR gamma . Conclusion: PPAR gamma is able to modulate the rate of neural differentiation of mouse embryonic stem cells.