An efficient and simple method for site-selective modification of O super(6)-methyl-2'-deo xyguanosine in DNA

O super(6)-Methyl-2'-deoxyg uanosine (O super(6)-Me-dG) is a mutagenic nucleotide in DNA. O super(6)-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O super(6)-Me-dG with...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 48; no. 33; pp. 3969 - 3971
Main Authors Onizuka, Kazumitsu, Nishioka, Takamasa, Li, Zhichun, Jitsuzaki, Daichi, Taniguchi, Yosuke, Sasaki, Shigeki
Format Journal Article
LanguageEnglish
Published 01.03.2012
Subjects
Biotin
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Summary:O super(6)-Methyl-2'-deoxyg uanosine (O super(6)-Me-dG) is a mutagenic nucleotide in DNA. O super(6)-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O super(6)-Me-dG with the fluorescent FAM or biotin group viaclick chemistry has permitted the sensitive and selective detection of O super(6)-Me- dG in DNA.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc17621a