A Rapid and Simple Method of Evaluating the Dimeric Tendency of Fluorescent Proteins in Living Cells Using a Truncated Protein of Importin I- as Fusion Tag

Enhanced green fluorescent protein (EGFP) and its yellow variant (Venus) are weakly dimeric under physiological conditions. We designed a simple method to evaluate the dimeric tendency of fluorescent proteins in living mammalian cells. A novel single mutation, A206L, interfering with the hydrophobic...

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Published inBioscience, biotechnology, and biochemistry Vol. 76; no. 2; pp. 388 - 390
Main Authors NAKAGAWA, Chika, NISHIMURA, Shigenori, SENDA-MURATA, Kaori, SUGIMOTO, Kenji
Format Journal Article
LanguageEnglish
Published 01.01.2012
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Summary:Enhanced green fluorescent protein (EGFP) and its yellow variant (Venus) are weakly dimeric under physiological conditions. We designed a simple method to evaluate the dimeric tendency of fluorescent proteins in living mammalian cells. A novel single mutation, A206L, interfering with the hydrophobic interactions of the dimer interface in Venus, contributed to its monomerization, and was as effective as the A206K mutation in this assay.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0916-8451
1347-6947