Agaricus blazei as a Substrate for the Production of [Beta]-1,3-Glucanase by Trichoderma harzianum Rifai

Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2^sup 2^-central composite factorial design was developed using the time of culture (x^sub 1^/day) and Agaricus blaz...

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Bibliographic Details
Published inFood technology and biotechnology Vol. 49; no. 3; p. 341
Main Authors Carneiro, Andréia A J, Giese, Ellen C, Barbosa, Aneli M, Gomes, Eleni, Da Silva, Roberto
Format Journal Article
LanguageEnglish
Published Zagreb Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet 01.07.2011
Subjects
Bacteria
Biomass
Carbohydrates
Enzymes
Glucose
Microbiology
Optimization techniques
Studies
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Summary:Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2^sup 2^-central composite factorial design was developed using the time of culture (x^sub 1^/day) and Agaricus blazei extract concentration (x^sub 2^/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai. [PUBLICATION ABSTRACT]
ISSN:1330-9862
1334-2606