Importin-[Beta] negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores

• Published in: The Journal of cell biology Vol. 196; no. 4; p. 435
• Main Authors: Roscioli, Emanuele, Di Francesco, Laura, Bolognesi, Alessio, Giubettini, Maria, Orlando, Serena, Harel, Amnon, Schininà, Maria Eugenia, Lavia, Patrizia
• Format: Journal Article
• Language: English
• Published: New York Rockefeller University Press 20.02.2012
• Subjects: Amino acids; Binding sites; Cell division; Molecular biology; Proteins
• ISSN: 0021-9525; 1540-8140

Importin-β is the main vector for interphase nuclear protein import and plays roles after nuclear envelope breakdown. Here we show that importin-β regulates multiple aspects of mitosis via distinct domains that interact with different classes of proteins in human cells. The C-terminal region (which binds importin-α) inhibits mitotic spindle pole formation. The central region (harboring nucleoporin-binding sites) regulates microtubule dynamic functions and interaction with kinetochores. Importin-β interacts through this region with NUP358/RANBP2, which in turn binds SUMO-conjugated RANGAP1 in nuclear pores. We show that this interaction continues after nuclear pore disassembly. Overexpression of importin-β, or of the nucleoporin-binding region, inhibited RANGAP1 recruitment to mitotic kinetochores, an event that is known to require microtubule attachment and the exportin CRM1. Co-expressing either importin-β-interacting RANBP2 fragments, or CRM1, restored RANGAP1 to kinetochores and rescued importin-β-dependent mitotic dynamic defects. These results reveal previously unrecognized importin-β functions at kinetochores exerted via RANBP2 and opposed by CRM1. [PUBLICATION ABSTRACT]