Effect of Copper on the Expression of TGF-[beta] in Incubated Chondrocytes of Newborn Pigs

• Published in: Biological trace element research Vol. 143; no. 3; p. 1461
• Main Authors: Zhu, Xiaoyan, Wang, Jianguo, Xie, Guanghong, Feng, Haihua, Li, Xiaobing, Liu, Lei, Wang, Xiaoxu, Li, Dongna, Liu, Zhaoxi, Qian, Jian, Wang, Zhe, Liu, Guowen
• Format: Journal Article
• Language: English
• Published: Clifton Springer Nature B.V 01.12.2011
• Subjects: Animal reproduction; Copper; Culture media; Gene expression; Hogs
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-011-8966-4

This experiment was conducted to measure the effect of copper supplementation on TGF-β gene expression in chondrocytes of newborn pigs. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. Total RNA was isolated from chondrocytes, and TGF-β cDNA was synthesized, amplified, and sequenced. The expression level of TGF-β was examined by reverse transcription polymerase chain reaction. The results showed that the sequence of the cloned TGF-β gene was 99.4% identical to that in GenBank. The expression of TGF-β increased in culture media added with final concentration of 15.6, 31.2, and 62.5 μmol/L copper. In this study, the optimal copper concentration and optimal culture time for the highest level of TGF-β expression were 31.2 μmol/L and 48 h, respectively. [PUBLICATION ABSTRACT]