Limitations of Direct Methods and the Reference Method for Measuring HDL and LDL Cholesterol/In Reply
The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B, especially in samples from patients with marked hypertriglyceridemia. [...] the way this study was carried out is biased and puts the direct...
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| Published in | Clinical chemistry (Baltimore, Md.) Vol. 57; no. 7; p. 1081 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington
Oxford University Press
01.07.2011
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B, especially in samples from patients with marked hypertriglyceridemia. [...] the way this study was carried out is biased and puts the direct measurements at a considerable disadvantage, especially for samples obtained from individuals with disease, who are more likely to have marked hypertriglyceridemia or a variety of other rare inborn errors of lipoprotein metabolism often seen at the NIH. [...] in our study design, the direct HDL-C and LDL-C methods were evaluated for their ability to produce results that agreed with the bestavailable reference measurement procedure. |
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| AbstractList | The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B, especially in samples from patients with marked hypertriglyceridemia. [...] the way this study was carried out is biased and puts the direct measurements at a considerable disadvantage, especially for samples obtained from individuals with disease, who are more likely to have marked hypertriglyceridemia or a variety of other rare inborn errors of lipoprotein metabolism often seen at the NIH. [...] in our study design, the direct HDL-C and LDL-C methods were evaluated for their ability to produce results that agreed with the bestavailable reference measurement procedure. |
| Author | Schaefer, Ernst J Myers, Gary L Ai, Masumi Miller, W Greg Remaley, Alan T Otokozawa, Seiko |
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| Copyright | Copyright American Association for Clinical Chemistry Jul 2011 |
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| Snippet | The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B,... |
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| SubjectTerms | Apolipoproteins Cardiovascular disease Cholesterol Clinical medicine Diet Low density lipoprotein Manganese Methods Plasma Studies |
| Title | Limitations of Direct Methods and the Reference Method for Measuring HDL and LDL Cholesterol/In Reply |
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