Limitations of Direct Methods and the Reference Method for Measuring HDL and LDL Cholesterol/In Reply

The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B, especially in samples from patients with marked hypertriglyceridemia. [...] the way this study was carried out is biased and puts the direct...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 57; no. 7; p. 1081
Main Authors Schaefer, Ernst J, Otokozawa, Seiko, Ai, Masumi, Miller, W Greg, Myers, Gary L, Remaley, Alan T
Format Journal Article
LanguageEnglish
Published Washington Oxford University Press 01.07.2011
Subjects
Apolipoproteins
Cardiovascular disease
Cholesterol
Clinical medicine
Diet
Low density lipoprotein
Manganese
Methods
Plasma
Studies
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Summary:The CDC uses the ultracentrifugation step because heparin-manganese chloride precipitation does not always remove all lipoproteins containing apolipoprotein B, especially in samples from patients with marked hypertriglyceridemia. [...] the way this study was carried out is biased and puts the direct measurements at a considerable disadvantage, especially for samples obtained from individuals with disease, who are more likely to have marked hypertriglyceridemia or a variety of other rare inborn errors of lipoprotein metabolism often seen at the NIH. [...] in our study design, the direct HDL-C and LDL-C methods were evaluated for their ability to produce results that agreed with the bestavailable reference measurement procedure.
ISSN:0009-9147
1530-8561