CDK5RAP2 stimulates microtubule nucleation by the [gamma]-tubulin ring complex

CDK5RAP2 is a human microcephaly protein that contains a ...-tubulin complex (...-TuC)-binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are ...-TuC-tethering proteins. In this study, we show that this domain within CDK5RAP2 associat...

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Bibliographic Details
Published inThe Journal of cell biology Vol. 191; no. 6; p. 1089
Main Authors Choi, Yuk-Kwan, Liu, Pengfei, Sze, Siu Kwan, Dai, Chao, Qi, Robert Z
Format Journal Article
LanguageEnglish
Published New York Rockefeller University Press 13.12.2010
Subjects
Binding sites
Biochemistry
Insects
Mutation
Proteins
Ribonucleic acid
RNA
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Summary:CDK5RAP2 is a human microcephaly protein that contains a ...-tubulin complex (...-TuC)-binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are ...-TuC-tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the ...-tubulin ring complex (...-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the ...-TuRC-mediated nucleation activator (...-TuNA). ...-TuNA but not its ...-TuC-binding-deficient mutant stimulates microtubule nucleation by purified ...-TuRC in vitro and induces extensive, ...-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. ...-TuRC bound to ...-TuNA contains NME7, FAM128A/B, and actin in addition to ...-tubulin and GCP2-6. RNA interference-mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although ...-TuRC assembly is unaffected. Collectively, these results suggest that the ...-TuNA found in CDK5RAP2 has regulatory functions in ...-TuRC-mediated microtubule nucleation. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0021-9525
1540-8140