Aerobic exercise training improves skeletal muscle function and Ca^sup 2+^ handling-related protein expression in sympathetic hyperactivity-induced heart failure

• Published in: Journal of applied physiology (1985) Vol. 109; no. 3; p. 702
• Main Authors: Bueno, C R, Ferreira, J C B, Pereira, M G, Bacurau, A V N, Brum, P C
• Format: Journal Article
• Language: English
• Published: Bethesda American Physiological Society 01.09.2010
• Subjects: Biochemistry; Calcium; Exercise; Heart failure; Musculoskeletal system; Proteins; Rodents
• ISSN: 8750-7587; 1522-1601

The cellular mechanisms of positive effects associated with aerobic exercise training on overall intrinsic skeletal muscle changes in heart failure (HF) remain unclear. We investigated potential ... abnormalities in skeletal muscles comprising different fiber compositions and investigated whether aerobic exercise training would improve muscle function in a genetic model of sympathetic hyperactivity-induced HF. A cohort of male 5-mo-old wild-type (WT) and congenic α.../α... adrenoceptor knockout (ARKO) mice in a C57BL/6J genetic background were randomly assigned into untrained and trained groups. Exercise training consisted of a 8-wk running session of 60 min, 5 days/wk (from 5 to 7 mo of age). After completion of the exercise training protocol, exercise tolerance was determined by graded treadmill exercise test, muscle function test by Rotarod, ambulation and resistance to inclination tests, cardiac function by echocardiography, and ... handling-related protein expression by Western blot. α.../α...ARKO mice displayed decreased ventricular function, exercise intolerance, and muscle weakness paralleled by decreased expression of sarcoplasmic ... release-related proteins [α...-, α...-, and β...-subunits of dihydropyridine receptor (DHPR) and ryanodine receptor (RyR)] and ... reuptake-related proteins [sarco(endo)plasmic reticulum ...-ATPase (SERCA)1/2 and .../... exchanger (NCX)] in soleus and plantaris. Aerobic exercise training significantly improved exercise tolerance and muscle function and reestablished the expression of proteins involved in sarcoplasmic ... handling toward WT levels. We provide evidence that ... handling-related protein expression is decreased in this HF model and that exercise training improves skeletal muscle function associated with changes in the net balance of skeletal muscle ... handling proteins. (ProQuest: ... denotes formulae/symbols omitted.)