Improvement of intracellular [beta]-galactosidase production in fed-batch culture of Kluyveromyces fragilis

Four fed-batch control strategies were evaluated to improve the specific lactase activity of Kluyveromyces fragilis. Control strategies tested included DO-stat control, exponential feeding, exponential feeding with manual feedback control and corrected feed-forward control. Each was implemented with...

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Bibliographic Details
Published inBiotechnology letters Vol. 23; no. 11; p. 845
Main Authors Nor, Zairossani M, Tamer, Melih I, Mehrvar, Mehrab, Scharer, Jeno M, Moo-young, Murray, Jervis, Eric J
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.06.2001
Subjects
Dissolved oxygen
pH sensors
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Summary:Four fed-batch control strategies were evaluated to improve the specific lactase activity of Kluyveromyces fragilis. Control strategies tested included DO-stat control, exponential feeding, exponential feeding with manual feedback control and corrected feed-forward control. Each was implemented with standard sensors (i.e., temperature, dissolved oxygen and pH sensors) commonly installed in fermenters. The highest specific activity was obtained using the corrected feed-forward control strategy, a strategy incorporating a novel method for on-line estimation of specific growth rate. The control strategy was able to operate effectively to a final cell density of 69 g dry wt l^sup -1^ with a specific lactase activity of 2 U mg^sup -1^ cell dry wt.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010526831985