Dysfunction of infiltrating cytotoxic CD8+ T cells within the graft promotes murine kidney allotransplant tolerance

Tolerance of mouse kidney allografts arises in grafts that develop regulatory tertiary lymphoid organs (rTLOs). Singlecell RNA-seq (scRNA-seq) data and adoptive transfer of alloreactive T cells after transplantation showed that cytotoxic CD8+ T cells are reprogrammed within the accepted graft to an...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of clinical investigation Vol. 134; no. 16; pp. 1 - 18
Main Authors Yokose, Takahiro, Szuter, Edward S, Rosales, Ivy, Guinn, Michael T, Liss, Andrew S, Baba, Taisuke, Ruddy, David A, Piquet, Michelle, Azzi, Jamil, Cosimi, A Benedict, Russell, Paul S, Madsen, Joren C, Colvin, Robert B, Alessandrini, Alessandro
Format Journal Article
LanguageEnglish
Published Ann Arbor American Society for Clinical Investigation 01.08.2024
Subjects
Adoptive transfer
Allografts
CD8 antigen
Cytotoxicity
Dendritic cells
Flow cytometry
Gene expression
Genotype & phenotype
Graft rejection
Immunological tolerance
Kidney transplantation
Kidneys
Lymphocytes
Lymphocytes T
Phenotypes
Proteins
Spleen
Tumors
Xenografts
Online AccessGet full text

Cover

More Information
Summary:Tolerance of mouse kidney allografts arises in grafts that develop regulatory tertiary lymphoid organs (rTLOs). Singlecell RNA-seq (scRNA-seq) data and adoptive transfer of alloreactive T cells after transplantation showed that cytotoxic CD8+ T cells are reprogrammed within the accepted graft to an exhausted/regulatory-like phenotype mediated by IFN-y. Establishment of rTLOs was required because adoptive transfer of alloreactive T cells prior to transplantation results in kidney allograft rejection. Despite the presence of intragraft CD8+ cells with a regulatory phenotype, they were not essential for the induction and maintenance of kidney allograft tolerance since renal allotransplantation into CD8-K0 recipients resulted in acceptance and not rejection. Analysis of scRNA-seq data from allograft kidneys and malignant tumors identified similar regulatory-1 ike cell types within the T cell clusters and trajectory analysis showed that cytotoxic CD8+ T cells are reprogrammed into an exhausted/regulatory-like phenotype intratu mo rally. Induction of cytotoxic CD8+ T cell dysfunction of infiltrating cells appears to be a beneficial mechanistic pathway that protects the kidney allotransplant from rejection through a process we call "defensive tolerance." This pathway has implications for our understanding of allotransplant tolerance and tumor resistance to host immunity.
ISSN:0021-9738
1558-8238
DOI:10.1172/JCI183501