A COMBINED BIOMARKER APPROACH IN THE DIAGNOSIS OF COLORECTAL CANCER

Objectives: Colorectal cancer (CRC) is a tumor with a constantly increasing incidence. Among the diagnostic approaches studied, biomarker combinations have so far performed better than single biomarkers. The present study aims to investigate the potential contribution of several such combinations to...

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Published inRomanian archives of microbiology and immunology Vol. 82; no. 3; pp. 178 - 186
Main Authors Farc, Ovidiu, Zaharie, Florin, Ţăulean, Roman, Vălean, Dan, Neagoe, Ioana Berindan, Budisan, Liviuta, Zănoagă, Oana, Lucaci, Elvira, Georgiu, Aurelian, Cristea, Victor
Format Journal Article
LanguageEnglish
Published Bucharest Institutul Cantacuzino 01.07.2023
Subjects
Biomarkers
Cell adhesion & migration
Cell adhesion molecules
Colorectal cancer
Colorectal carcinoma
Cytokines
Diagnosis
Enzyme-linked immunosorbent assay
Enzymes
Gastroenterology
Hepatology
Inflammatory diseases
Intercellular adhesion molecule 1
Interleukin 8
Matrilysin
Matrix metalloproteinase
Metalloproteinase
P-selectin
Regression analysis
Serum levels
Software
Tumors
United States > US
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Summary:Objectives: Colorectal cancer (CRC) is a tumor with a constantly increasing incidence. Among the diagnostic approaches studied, biomarker combinations have so far performed better than single biomarkers. The present study aims to investigate the potential contribution of several such combinations to the noninvasive diagnosis of CRC. Methods: Serum levels of a cytokine (IL-8), two cell adhesion molecules (ICAM-1 and P-selectin-Psel) and a matrix-metalloproteinase (MMP-7) were measured by ELISA (Enzyme-Linked Immunosorbent Assay) in thirty-one CRC patients and thirty-three healthy controls. The biomarker potential of combinations between these molecules was tested by logistic regression. Results: The tested combinations had good biomarker potential, with AUCs (Areas under the curve) between 0.841 and 0.857, sensitivities (Sn) ranging from 0.77 to 0.87, and specificities (Sp) between 0.7 and 0.85. The best performance was recorded for the combination IL8-Psel-MMP7, with an AUC of 0.857, Sn of 0.85, and Sp of 0.80. For the early stages of CRC, the same combination had an AUC of 0.850, Sn of 0.81, and Sp of 0.85. Conclusions: The findings of this study underscore the significant potential of biomarker combinations in the diagnosis of CRC, particularly in the early stages. The IL8-Psel-MMP7 combination, in particular, demonstrates the highest biomarker capability, offering a promising avenue for non-invasive diagnosis of CRC.
ISSN:1222-3891
2601-9418