Cloning, expression, and function of Hc KAN4 gene of kenaf in flavonoid synthesis

MYB-like transcription factor KAN4（KANADI4） plays an important role in flavonoid synthesis and fiber development in kenaf. In this study, the variety "Fuhong 952" was used as the experimental material to clone and analyze the relative expression pattern of HcKAN4 genes, and to investigate...

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Bibliographic Details
Published inZuo wu xue bao Vol. 50; no. 3; p. 645
Main Authors Wu, Fa-Xuan, Li, Qin, Yang, Xin, Li, Xin-Gen, Xu, Jian-Tang, Tao, Ai-Fen, Fang, Ping-Ping, Qi, Jian-Min, Zhang, Li-Wu
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2024
Subjects
Amino acids
Biosynthesis
Cloning
Flavonoids
Gene expression
Gene silencing
Genes
Kenaf
Open reading frames
Phylogeny
Plant growth
Plant tissues
Transcription factors
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Summary:MYB-like transcription factor KAN4（KANADI4） plays an important role in flavonoid synthesis and fiber development in kenaf. In this study, the variety "Fuhong 952" was used as the experimental material to clone and analyze the relative expression pattern of HcKAN4 genes, and to investigate the effect of TRV-VIGS-induced silencing of HCKAN4 gene on the expression of key enzyme genes in flavonoid synthesis pathway in kenaf, the variety 'Fuhong 952' was used as the experimental material. Gene cloning showed that the total length of ORF of Hc KAN4 gene was 966 bp, encoding 322 amino acids and containing a conserved domain of MYB. Phylogenetic tree revealed that it was closely related to KAN4s of Arabidopsis and Hibiscus. The relative expression pattern indicated that the gene was expressed in different plant tissues, and the transcription level increased with plant growth in kenaf.VIGS-induced gene silencing revealed that the transcription level of 6 HcKAN4 individuals was significantly down-regulated, indicating
ISSN:0496-3490