Establishment of a TLC Identification Method for Ensete wilsonii

• Published in: Plant diseases and pests Vol. 15; no. 1; pp. 4 - 6
• Main Authors: Su, Yongjing, Xie, Ao, Liang, Wenwen, Zeng, Fanglin, Wen, Haicheng, Wei, Wei
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.02.2024
• Subjects: Aluminum; Angina pectoris; Chinese medicine; Chromatography; Dichloromethane; Ethanol; Hypertension; Identification; Investigations; Methods; Quality control; Silica gel; Solvents; Sulfuric acid; Ultrasonic imaging
• ISSN: 2152-3932
• DOI: 10.19579/j.cnki.plant-d.p.2024.01.002

Modern studies have shown that E. wilsonii has pharmacological effects such as treating hypertension and lowering blood sugar ' 6 , and the plants of this species contain volatile oil171 , phenols181 , alkaloids191 , ß-sitosterol1'01 and other chemical components. [...]the above compounds can be used as index components in the subsequent evaluation of the quality standard of E. wilsonii. [...]there is no perfect and feasible quality control system for the quality control of E. wilsonii. [...]this paper identified E. wilsonii by thin layer chromatography (TLC) , in order to provide a reference for the establishment of the quality standard of E. wilsonii. 2 Materials 2 . The examination results under an ultraviolet lamp at 365 nm showed that various spots had clear size and color at the developing distance of 15 cm. [...]the developing distance of 15 cm was selected. 4 TLC identification In summary, TLC identification method for E. wilsonii was determined as follows: the test solution and reference solution were prepared according to the method described in Section 3.1.1; 5 p,L of each batch of test solution and 3 pL of reference solution were dotted on the same silica gel G plate, and developed with dichloromethane-toluene-methanol (10 I 5 I 1.5) as the developing solvent; the plate was then taken out and dried with hot blast, and then developed color by spraying 10% sulfuric acid ethanol solution. [...]the TLC identification method established in this paper is simple and easy to operate, economical and practical, with obvious qualitative characteristics and intuitive results, and can provide a certain reference for the quality evaluation standard of E. wilsonii.