Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murineimmunoglobulin G by immobilized Ni(II) ions

Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (u...

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Bibliographic Details
Published inPolymer bulletin (Berlin, Germany) Vol. 61; no. 2; pp. 147 - 156
Main Authors Kráčalíková, Kateřina, Bleha, Miroslav
Format Journal Article
LanguageEnglish
Published Heidelberg Springer Nature B.V 01.08.2008
Subjects
Chelation
Copolymers
Copper
IgG antibody
Membranes
Nickel
Scavenging
Sorbents
Sorption
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Summary:Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (up to 1.3 mmol/g). The potential use of immobilized Ni(II)-IDA complexes for sorption of murine immunoglobulin G by the immobilized metal affinity (IMA) method was tested. The sorption behaviour was characterized by a Langmuir-Freundlich adsorption isotherm. It was shown that the chelating membranes could be used for the sorption of low amounts of protein.
ISSN:0170-0839
1436-2449
DOI:10.1007/s00289-008-0940-8