Creation of doubled haploid in wheat using distant hybridization and unreduced gamete genes

The union of unreduced female and male gametes leads to spontaneous chromosome doubling,which is not only an important way of the origin of polyploid species but also an essential tool for enhancing crop doubled haploid breeding efficiency.A major quantitative trait locus QTug.sau-3B responsible for...

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Published inZuo wu xue bao Vol. 49; no. 12; p. 3154
Main Authors Liu, Xiao-Juan, Liu, Xin, Zhang, Ming-Hu, Hao, Ming, Ning, Shun-Zong, Yuan, Zhong-Wei, Huang, Lin, Liu, Deng-Cai, Zhang, Lian-Quan
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2023
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Summary:The union of unreduced female and male gametes leads to spontaneous chromosome doubling,which is not only an important way of the origin of polyploid species but also an essential tool for enhancing crop doubled haploid breeding efficiency.A major quantitative trait locus QTug.sau-3B responsible for unreduced gametes formation was discovered in tetraploid Triticum turgidum wheat and was further transferred to elite common wheat lines using synthetic hexaploid wheat as a bridge.This objective of this study is to make wheat/wheat F1 hybrids between the elite lines with this gene region and commercial cultivars and to evaluate the efficiency of QTug.sau-3B gene leading to spontaneous chromosome doubling.Wheat/wheat F1 hybrids and their parents were pollinated with the fresh pollen of Imperata cylindrica to produce wheat haploid through chromosome elimination of I.cylindrica.Doubled haploid(DH) were then developed by spontaneous chromosome doubling in haploids because of the union of unreduced female and male gam
ISSN:0496-3490