Optimization of Extraction and Antioxidant Activities of Kaempferia parviflora Rhizome

Traditional herbal plants contribute significantly to human health owing to the abundance of appealing bioactive compounds. Among them is Kaempferia parviflora (KP) rhizome, a widely used traditional medication that attracted diverse attention in East and Southeast Asia. The KP extract is rich in fl...

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Bibliographic Details
Published inAsia-Pacific journal of molecular biology and biotechnology Vol. 31; p. 62
Main Authors Sanadi, Adibah Hayati Mohd, Mutalib, Maisarah Abdul
Format Journal Article
LanguageEnglish
Published Kuala Lumpur Malaysian Society for Molecular Biology and Biotechnology 01.06.2023
Subjects
Antioxidants
Aquatic plants
Bioactive compounds
Biological effects
Cocoa
Ethanol
Flavones
Flavonoids
Free radicals
Kaempferia parviflora
Optimization
Phenolic compounds
Phenols
Rhizomes
Room temperature
Scavenging
Solvents
Subgroups
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Summary:Traditional herbal plants contribute significantly to human health owing to the abundance of appealing bioactive compounds. Among them is Kaempferia parviflora (KP) rhizome, a widely used traditional medication that attracted diverse attention in East and Southeast Asia. The KP extract is rich in flavones derivatives, a subgroup of flavonoids resulting in astonishing biological activities. Nonetheless, the antioxidant activity of the KP extract remained a point of contention as the free radicals scavenging activity of the crude extract is influenced by multiple factors. Therefore, this study aims to optimise several parameters and extraction methods to maximise extraction yields, phenolic and flavonoid content and achieve the highest possible levels of antioxidant activity in the KP extracts. The KP rhizomes were extracted by maceration techniques using different solvent types (ethanol and methanol). The antioxidant activities of KP extract were optimised for different solvent concentrations (70 and 90% V/V), temperature degrees (room temperature and 60 °C), and sample forms (fresh and dried KP) using a uv-vis spectrophotometer. All the samples were subjected to Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and 2,2-Diphenyl-1picrylhydrazyl (DPPH) assay. Increasing ethanol concentrations up to 90% enhanced the yield (27.18%), TPC (430.14 ± 19.25), and TFC (694.32 ± 7.33), but not for DPPH where 90% of methanolic extraction exhibited the highest DPPH scavenging activities for both fresh (94%) and dried (86%) KP samples. Kaempferia parviflora extract is highly valuable for its antioxidant activity under optimal conditions. Extraction methods play a major role in controlling the phytochemical properties of KP rhizome extracts. This study found that 90% of ethanol concentrations affected KP's antioxidant properties by altering antioxidant components and their activities.
ISSN:0128-7451