Genome-wide identification and in silico characterization of patatin-related phospholipase A (pPLA) gene family in Gossypium arboreum and Gossypium barbadense

• Published in: NeuroQuantology Vol. 20; no. 16; p. 3308
• Main Authors: Parveen, Reshma, Vaish, Swati, Singh, Nootan, Gupta, Divya, Basantani, Mahesh Kumar
• Format: Journal Article
• Language: English
• Published: Bornova Izmir NeuroQuantology 01.01.2022
• Subjects: Chloroplasts; Clustering; Cytoplasm; Genes; Mitochondria; Proteins
• ISSN: 1303-5150
• DOI: 10.48047/NQ.2022.20.16.NQ880340

Patatin-related phospholipaseA (pPLA) were found to be involved in diverse plant physiological and metabolic processes, ranging from normal organ development to managing biotic and abiotic stresses. The present study led to the identification and characterization of pPLA gene family in two agriculturally important Gossypium species viz. G. arboreum and G. barbadense. A total of 22 and 36 pPLA genes were identified in G. arboreum and G. barbadense respectively. The identified pPLA genes were categorized in three canonical groups; Group I, Group II and Group III. The established patatin domain was found at the N-terminus with the esterase and anion box. The molecular weight varied across the groups. Group I pPLA proteins were 65 to 140 kDa whereas Group II and Group III were in the range of 40 to 50 kDa. The isoelectric point (pI) was in the range of 5 to 9. Subcellular localization prediction was noticed to be group specific as Group I proteins of G. arboreum and G. barbadense were centrally localized in the nucleus, Group II and Group III proteins were mainly found in cytoplasm, chloroplast and mitochondria. Gene architecture analysis revealed the exon numbers in the range of 2 to 18. MEME analysis identified many group specific motifs and many common motifs. Phylogenetic analysis led to the clustering of the pPLAs in the three different clades representing Group I, II and III. Segmental duplication was driving force for pPLA gene family expansion in G. arboreum and G. barbadense. The results of current study can be considered as a platform for molecular and functional characterization of this gene family in G. arboreum and G. barbadense under developmental and stress conditions.