Interface Self-Referenced Dynamic Full-Field Optical Coherence Tomography

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D samples. However, D-FFOCT suffers from fringe artefacts whe...

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Bibliographic Details
Published inarXiv.org
Main Authors Monfort, Tual, Azzollini, Salvatore, Tasnim Ben Yacoub, Audo, Isabelle, Reichman, Sacha, Grieve, Kate, Thouvenin, Olivier
Format Paper
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 17.02.2023
Subjects
Optical Coherence Tomography
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Summary:Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D samples. However, D-FFOCT suffers from fringe artefacts when imaging nearby reflective surfaces and is highly sensitive to vibrations. Here, we present interface Self-Referenced (iSR) D-FFOCT, an alternative configuration to D-FFOCT that takes advantage of the presence of the sample coverslip in between the sample and the objective by using it as a defocused reference arm, thus avoiding the aforementioned artefacts. We demonstrate the ability of iSR D-FFOCT to image 2D fibroblast cell cultures, which are among the flattest mammalian cells.
ISSN:2331-8422