Genome editing of BnaMPK6 gene by CRISPR/Cas9 for loss of salt tolerance in Brassica napus L

Brassica napus L. is an important oil crop. MPK6（Mitogen-Activated Protein Kinases 6） is activated by various stresses to control plant stress tolerance, which is the key gene in response to abiotic stresses. However, the function of MAPK6 in B. napus stress tolerance is still unclear. Gene structur...

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Bibliographic Details
Published inZuo wu xue bao Vol. 49; no. 2; p. 321
Main Authors Zhang, Wen-Xuan, Liang, Xiao-Mei, Dai, Cheng, Wen, Jing, Yi, Bin, Tu, Jin-Xing, Shen, Jin-Xiong, Fu, Ting-Dong, Ma, Chao-Zhi
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2023
Subjects
Amino acid sequence
Brassica
Brassica napus
CRISPR
Editing
Genetic improvement
Genome editing
Genomes
Kinases
MAP kinase
Mutants
Oilseeds
Plant stress
Protein kinase
Protein structure
Proteins
Rape plants
Reactive oxygen species
Salinity tolerance
Salt tolerance
Sequence analysis
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Summary:Brassica napus L. is an important oil crop. MPK6（Mitogen-Activated Protein Kinases 6） is activated by various stresses to control plant stress tolerance, which is the key gene in response to abiotic stresses. However, the function of MAPK6 in B. napus stress tolerance is still unclear. Gene structure and protein sequence analysis showed that MPK6 gene structures were similar, and all proteins had the same STKc＿TEY＿MAPK domain in Brassica. To explore the potential role of BnaMPK6, the BnaMPK6 mutants were generated by CRISPR/Cas9 genome editing technology. Two quadruple mutants, cr-bnampk6-13-1 and cr-bnampk6-49-1, were obtained. cr-bnampk6 mutant lines were hypersensitive to salt treatment（100 mmol L–1 and 150 mmol L–1）, the growth of the mutant was strongly inhibited after salt treatment, and the plant height and fresh weight were significantly lower than those of wild-type plants, but there was no significant difference in root length. In addition, reactive oxygen species（ROS）, malondialdehyde（MDA）, and fre
ISSN:0496-3490