Ixodes ricinus tick bacteriome alterations based on a climatically representative survey in Hungary

• Published in: bioRxiv
• Main Authors: Tóth, Adrienn Gréta, Farkas, Róbert, Papp, Márton, Kilim, Oz, Haeun, Yun, Makrai, László, Maróti, Gergely, Gyurkovszky, Mónika, Krikó, Eszter, Solymosi, Norbert
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.12.2022
• Subjects: Arachnids; Arsenophonus; Bacillus; Candidatus Midichloria; Climate change; Climatic conditions; Curtobacterium; Developmental stages; Ixodes ricinus; Metagenomics; Microbiomes; Next-generation sequencing; Pseudomonas; Public health; Relative abundance; Rhodococcus; Rickettsia; Sampling; Sphingomonas; Staphylococcus; Statistical analysis; Vectors; Vectors (Biology); Wolbachia; Hungary
• DOI: 10.1101/2022.10.15.512391

Background: The microbial communities of disease vectors may represent a key feature in several biological functions and thus deserve special attention in light of climate change and the consequent need to develop novel control strategies. Nevertheless, vector-borne microbial networks are still poorly understood. Assessing vectors' microbial interactions and climatic dependencies may contribute to better-estimating pathogen transmission characteristics, public health risks and the urgency for control steps to be taken. Methods: In a climatically representative country-wide survey, Ixodes ricinus ticks were collected from 17 locations in Hungary. Using shotgun metagenomic sequencing, the bacteriome composition was analyzed by investigating the relationship between the abundances of nymphs and females in various climatic environments. Results: Bacterial composition on the genus level revealed a significant difference between the samples from females and nymphs. Within the core bacteriome, females and nymphs showed significant variation in the following genera: Arsenophonus, Bacillus, Candidatus Midichloria, Rhodococcus, Sphingomonas, Staphylococcus, Wolbachia. Among females, according totemperature strata, the following were found differentiating: Curtobacterium, Pseudomonas, Sphingomonas. There was no genus with a significant difference in precipitation categories for females. In the nymphs, Curtobacterium showed significant variation between temperature and Bacillus and Curtobacterium for various precipitation levels. Based on the full sample set, Arsenophonus and Wolbachia correlated positively which we assumed to have occurred due to the presence of I. hookeri. Conclusions: The composition of vector-borne bacteriome members showed significant alterations at sampling points with different climatic conditions and development stages of the tick hosts. Our findings not only pave the way towards understanding tick-borne bacterial networks and interdependencies but also shed light on the high potential for the presence of a possible biological tick control species, the tick parasitoid, Ixodiphagus hookeri based on related bacteriome patterns. The results of conscious tick microbiome assessment studies may contribute to precision tick control strategies of the future.Competing Interest StatementThe authors have declared no competing interest.Footnotes* The title was reworded, the abstract was extended, the introduction was shortened, and some results were moved into the supplementary information section.