INCREASING DIVERSITY AMONG LENS SPECIES FOR IMPROVING BIOLOGICAL NITROGEN FIXATION IN LENTIL

Exotic germplasm is a key resource for reintroducing genetic variability into cultivars. We evaluated 36 accessions from cultivated lentil (Lens culinaris Medik.) and six related wild species, inoculated with a commercial strain of Rhizobium leguminosarum bv. viciae under greenhouse conditions. The...

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Bibliographic Details
Published inbioRxiv
Main Authors Vargas, Ana, Gorim, Linda Y, Bett, Kirstin
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 16.10.2022
Subjects
Cultivars
Flowering
Genetic variability
Germplasm
Immunoglobulins
Inoculation
Nitrogen
Nitrogen fixation
Nodulation
Seeds
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Summary:Exotic germplasm is a key resource for reintroducing genetic variability into cultivars. We evaluated 36 accessions from cultivated lentil (Lens culinaris Medik.) and six related wild species, inoculated with a commercial strain of Rhizobium leguminosarum bv. viciae under greenhouse conditions. The objective was to explore Lens species and/or accessions that could contribute higher biological nitrogen fixation (BNF) ability to the lentil crop. A split plot design was used with either Rhizobium inoculation, added Nitrogen (N) or neither, as the main plots, and accessions in subplots randomized in blocks. Two repeats of the experiment were evaluated at flowering for N fixation and nodulation characters, and two subsequent experiments, with a subset of 14 accessions, were evaluated at maturity for seed production, seed quality and harvest index. Differences in phenotypic expression did not correspond to any particular Lens species. CDC Greenstar exhibited some of the highest N fixation values observed among the cultivars and also superior yield results compared to the added N treatment. Wild accessions, including IG 72643 (L. orientalis), displayed unique and multiple desirable characteristics compared to cultivars including indeterminate nodulation, higher N translocation, stable yield compared to added N treatment and exceptionally high protein concentration in seeds. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2022.10.12.510843