Microscopic-scale recording of brain neuronal electrical activity using a diamond quantum sensor

An important tool in the investigation of the early stages of neurodegenerative disease is the study of dissected living tissue from the brain of an animal model. Such investigations allow the physical structure of individual neurons and neural circuits to be probed alongside neuronal electrical act...

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Published inarXiv.org
Main Authors Nikolaj Winther Hansen, James Luke Webb, Troise, Luca, Olsson, Christoffer, Tomasevic, Leo, Brinza, Ovidiu, Achard, Jocelyn, Staacke, Robert, Kieschnick, Michael, Meijer, Jan, Thielscher, Axel, Hartwig Roman Siebner, Berg-Sørensen, Kirstine, Perrier, Jean-François, Huck, Alexander, Ulrik Lund Andersen
Format Paper
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 30.08.2022
Subjects
Axons
Biocompatibility
Brain
Circuits
Color centers
Diamond tools
Fluorescent dyes
Genetic modification
Image resolution
Pharmacology
Quantum sensors
Recording
Tetrodotoxin
Tissues
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Summary:An important tool in the investigation of the early stages of neurodegenerative disease is the study of dissected living tissue from the brain of an animal model. Such investigations allow the physical structure of individual neurons and neural circuits to be probed alongside neuronal electrical activity, disruption of which can shed light on the mechanisms of emergence of disease. Existing techniques for recording activity rely on potentially damaging direct interaction with the sample, either mechanically as point electrical probes or via intense focused laser light combined with highly specific genetic modification and/or potentially toxic fluorescent dyes. In this work, we instead perform passive, microscopic-scale recording of electrical activity using a biocompatible quantum sensor based on colour centres in diamond. We record biomagnetic field induced by ionic currents in mouse corpus callosum axons without direct sample interaction, accurately recovering signals corresponding to action potential propagation while demonstrating in situ pharmacology during biomagnetic recording through tetrodotoxin inhibition of voltage gated sodium channels. Our results open a promising new avenue for the microscopic recording of neuronal signals, offering the prospect of high resolution imaging of electrical circuits in the living mammalian brain.
ISSN:2331-8422