Preparation of highly specific wheat ATG8 antibody and its application in the detection of autophagy

Autophagy is an evolutionarily highly conserved lysosomal/vacuolar degradation pathway, which is of great significance for the body to adapt to various biotic or abiotic stresses and maintain its normal growth and development. Autophagy-related protein 8（ATG8） is the gold standard for detecting auto...

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Bibliographic Details
Published inZuo wu xue bao Vol. 48; no. 9; p. 2390
Main Authors Li, Yong-Bo, Cui, De-Zhou, Huang, Chen, Sui, Xin-Xia, Fan, Qing-Qi, Chu, Xiu-Sheng
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2022
Subjects
Amino acid sequence
Amino acids
Antibodies
Antigens
Autophagy
Excavation
Immunization
Leaves
Polyclonal antibodies
Polypeptides
Proteins
Rabbits
Roots
Seeds
Wheat
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Summary:Autophagy is an evolutionarily highly conserved lysosomal/vacuolar degradation pathway, which is of great significance for the body to adapt to various biotic or abiotic stresses and maintain its normal growth and development. Autophagy-related protein 8（ATG8） is the gold standard for detecting autophagy. At present, the lack of specific wheat ATG8 antibody has led to slow progress in the research of wheat autophagy. In this study, an 18-amino acid polypeptide of wheat ATG8 protein sequence was artificially synthesized as an antigen to immunize rabbits, and a highly specific wheat ATG8 polyclonal antibody was successfully obtained. The antibody can not only recognize the ATG8 protein bands in roots, leaves, and seeds of wheat, but also can detect autophagy structures in roots and leaves, and autophagy structures in wheat grains were detected for the first time.Besides eight typical types of ATG8a-h antibody found in wheat, a typical new ATG8 protein was detected by wheat ATG8 antibody developed in this study,
ISSN:0496-3490