Cervical Cancer and Next Generation Sequencing (NGS): A Systematic Review

Introduction: Cervical cancer is one of the most common types of cancer frequently reported in women worldwide. It is ranked as the second most occurring cancer in women worldwide. Next Generation Sequencing (NGS) is one of the emerging technologies that can to contribute towards precision medicine....

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Bibliographic Details
Published inAsia-Pacific journal of molecular biology and biotechnology Vol. 29; p. 108
Main Authors Hamid, Nazefah Abdul, Elias, Marjanu Hikmah, Nordin, Noraziah
Format Journal Article
LanguageEnglish
Published Kuala Lumpur Malaysian Society for Molecular Biology and Biotechnology 01.06.2021
Subjects
Cancer
Cervical cancer
Cervical carcinoma
Cervix
Criteria
DNA methylation
Genotyping
miRNA
Molecular modelling
Mutation
New technology
Next-generation sequencing
Precision medicine
Reviews
Systematic review
Viruses
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Summary:Introduction: Cervical cancer is one of the most common types of cancer frequently reported in women worldwide. It is ranked as the second most occurring cancer in women worldwide. Next Generation Sequencing (NGS) is one of the emerging technologies that can to contribute towards precision medicine. In this study, we aim to determine the use of NGS using cervical cancer research. Methods: This systematic review was done using PubMed as the database of searching. Keywords used is 'cervical cancer' AND 'Next Generation Sequencing'. The search limits were languages (English), dates (year from 2005 until 2021) and types of articles (original articles). Results: A total of 63 articles were found to fit the criteria. From the list, further inclusion criteria were used including original articles, using human samples and not on genotyping of the viruses. A total of 7 studies met the inclusion criteria and the full text was obtained for further analyses. The studies used CIN1, CIN2, CIN3 and cervical carcinoma obtained either as fresh tissues or FFPE. From these studies, four studies reported on CpG methylation, while only one study reported on the genes that are expressed, miRNA and mutations, respectively. Conclusion: As a conclusion, the data obtained from this systematic review is hoped to help us to understand the molecular mechanisms underlying progression in cervical cancer.
ISSN:0128-7451