A PILOT CASE-CONTROL STUDY: MICROBIOME ANALYSIS OF FECAL SAMPLES OF COLORECTALCANCER PATIENTS FROM CHILE AND ARGENTINA

Studies about the colorectal cancer (CRC) associated microbiome have mainly been conducted in developed countries with a high incidence of colorectal cancer. In the present work, we compare the CRC-associated microbiome of two developing countries with intermediate CRC incidence, Chile and Argentina...

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Bibliographic Details
Published inBAG. Journal of basic and applied genetics Vol. 32; p. 104
Main Authors Mayordomo, AC, Valladares, C Tapia, Wood, H, Young, C, Argüero, J, Balaguer, A Fuentes, Etcheverry, J Fuhr, Pavicic, W, Risk, M, Quirke, P, Melendez, L Contreras, Vaccaro, C, Piñero, TA
Format Journal Article
LanguageEnglish
Spanish
Published Buenos Aires Sociedad Argentina de Genetica 01.10.2021
Subjects
Bacteroidetes
Bioinformatics
Cancer
Cancer screening
Colorectal cancer
Colorectal carcinoma
Community structure
Developing countries
Feces
Firmicutes
LDCs
Medical screening
Microbiomes
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Summary:Studies about the colorectal cancer (CRC) associated microbiome have mainly been conducted in developed countries with a high incidence of colorectal cancer. In the present work, we compare the CRC-associated microbiome of two developing countries with intermediate CRC incidence, Chile and Argentina. Faecal samples from 10 healthy volunteers (HV) and 10 CRC patients from each country were collected using bowel cancer screening cards. Hiseq Illumina platform was used for V4 16SrRNA sequencing. Bioinformatics analysis was performed by Qiime2 and data was exported to determine taxa which differed significantly between groups using LEfSe. Alpha diversity was calculated and significance was assessed by the Kruskal-Wallis test. Weighted/Unweighted Unifrac distance was calculated and plotted as principal coordinate analysis plots. The significance of differences in beta diversity between groups was assessed by PERMANOVA analysis performed using the Adonis package for R. No significant differences in bacterial community structure and alpha diversity were detected. Statistical differences were observed in the beta diversity for unweighted UniFrac distances between the HV and CRC groups from each country. No differences at level of phylum in the relative abundance of Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria and Verrucomicrobia were detected. No significant differences in the ratio Firmicutes/Bacteroidetes were found. The LEfSe analysis showed significant differences in taxa level independent analysis between HV and CRC groups in each country. The present work is the first comparative study of the faecal CRC-microbiome in South American countries. Local projects are underway to expand the number of patients and validate the present results.
ISSN:1666-0390
1852-6233