First description of a Yersinia pseudotuberculosis clonal outbreak in France, confirmed using a new core genome multilocus sequence typing method

Yersinia pseudotuberculosis is an enteric pathogen causing mild enteritis that can lead to mesenteric adenitis and septicemia in elderly patients. Most cases are sporadic, but outbreaks have already been described in different countries. We report for the first time a Y. pseudotuberculosis clonal ou...

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Bibliographic Details
Published inbioRxiv
Main Authors Savin, Cyril, Anne-Sophie Le Guern, Chereau, Fanny, Guglielmini, Julien, Heuze, Guillaume, Demeure, Christian, Pizarro-Cerda, Javier
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.03.2022
Subjects
Adenitis
Contamination
Enteritis
Epidemiology
Genomes
Multilocus sequence typing
Nucleotide sequence
Outbreaks
Pseudotuberculosis
Septicemia
Yersinia pseudotuberculosis
France
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Summary:Yersinia pseudotuberculosis is an enteric pathogen causing mild enteritis that can lead to mesenteric adenitis and septicemia in elderly patients. Most cases are sporadic, but outbreaks have already been described in different countries. We report for the first time a Y. pseudotuberculosis clonal outbreak in France, that occurred in 2020. An epidemiological investigation pointed towards the consumption of tomatoes as the likely source of contamination. The Yersinia National Reference Laboratory (YNRL) developed a new cgMLST scheme with 1,921 genes specific to Y. pseudotuberculosis that identified the clustering of isolates associated to the outbreak and allowed to perform molecular typing in real time. In addition, this method allowed to retrospectively identify isolates belonging to this cluster from earlier in 2020. This method, which does not require specific bioinformatic skills, is now used systematically at the YNRL and proves to display an excellent discriminatory power and is available to the scientific community. Competing Interest Statement The authors have declared no competing interest. Footnotes * I added the supplementary data to the first version
DOI:10.1101/2022.03.23.485572