Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Accelerate Wound Healing

The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignosulfonates to confer antioxidation to tissue microenvironments and to deliver oxygen to accelerate vascularization and healing respon...

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Bibliographic Details
Published inbioRxiv
Main Authors Swathi Balaji, Short, Walker D, Padon, Benjamin W, Prajapati, Tanuj J, Farouk, Fayiz, Belgodere, Jorge A, Jimenez, Sarah E, Deoli, Naresh T, Guidry, Anna C, Green, Justin C, Kaul, Aditya, Son, Dongwan, Jung, Olivia S, Astete, Carlos E, Kim, Myungwoong, Jung, Jangwook P
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 20.03.2022
Subjects
Actin
Antioxidants
Biomaterials
Blood vessels
Collagen
Controlled release
Fibroblasts
Fibrosis
Gelatin
Inflammation
Lignin
Microenvironments
Nanoparticles
Oxygen
Smooth muscle
Sulfonation
Tissue engineering
Vascularization
Wound healing
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Summary:The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignosulfonates to confer antioxidation to tissue microenvironments and to deliver oxygen to accelerate vascularization and healing responses without causing inflammatory responses. The results from fibrosis array shows that thiolated lignosulfonate in methacrylated gelatin can effectively attenuate fibrotic responses of human dermal fibroblasts. Elemental analysis of oxygen releasing nanoparticles shows the positive incorporation of calcium peroxide. Composites including nanoparticles of lignosulfonate and calcium peroxide release around 0.05% oxygen per day at least for 7 days. Stiffness can be precisely modulated to avoid adverse inflammatory responses. Injection of lignin composites with oxygen generation nanoparticles enhanced the formation of blood vessels and promoted infiltration of alpha-smooth muscle actin+ fibroblasts over 7 days. At 30 days after surgery, the lignin composite with oxygen generating nanoparticles remodels the collagen architecture resembling to the reticular pattern of normal collagen and leave minimal scars. Thus, our study shows the potential of functionalized lignosulfonate for wound healing applications requiring balanced antioxidation and controlled release of oxygen. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2022.03.18.484913