Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea

Self-incompatibility（SI） refers to the characteristic that the pistil of a plant can recognize its own pollen and allogeneic pollen so as to inhibit the germination of its own pollen. PUB（Plant U-Box） proteins play an important role in plant stress resistance and signal transduction. In this study,...

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Published inZuo wu xue bao Vol. 48; no. 1; p. 108
Main Authors Xie, Qin-Qin, Zuo, Tong-Hong, Hu, Deng-Ke, Liu, Qian-Ying, Zhang, Yi-Zhong, Zhang, He-Cui, Zeng, Wen-Yi, Yuan, Chong-Mo, Zhu, Li-Quan
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2022
Subjects
Brassica
Brassica oleracea
Cloning
Cross-pollination
Cytoplasm
Domains
E coli
Gene expression
Germination
Incompatibility
Localization
Molecular weight
Plant reproduction
Plant stress
Pollen
Pollination
Proteins
Self-incompatibility
Sepals
Signal transduction
Stamens
Stigmas (botany)
Transcriptomes
Yeasts
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Summary:Self-incompatibility（SI） refers to the characteristic that the pistil of a plant can recognize its own pollen and allogeneic pollen so as to inhibit the germination of its own pollen. PUB（Plant U-Box） proteins play an important role in plant stress resistance and signal transduction. In this study, through the analysis of stigma transcriptome data of Brassica oleracea self-pollination at 0–60 minute（s）, a PUB protein coding gene BoPUB9 was screened and up-regulated by self-pollination. The BoPUB9 cDNA sequence was 1368 bp, gDNA of 1720 bp in length, containing one arm repeat domain and one U-box domain. The relative expression levels showed that BoPUB9 gene was expressed in different tissues of Brassica oleracea, and the relative expression level was the highest in sepals, followed by petals, stamens and stigmas, and relatively lower in pollen buds, which was consistent with the results of GUS staining. The relative expression level of BoPUB9 gene was up-regulated rapidly in self-pollination 0–30 minute（s）, t
ISSN:0496-3490