Identification of upstream regulators for mitogen-activated protein kinase 7 gene（BnMAPK7） in rapeseed（Brassica napus L.）

Mitogen-activated protein kinases（MAPKs） cascade plays a key role in plant growth and development, division, differentiation, apoptosis, and stress resistance. In this study, a 1612 bp promoter of C group BnMAPK7 gene, designated ProBnMAPK7, was cloned from Brassica napus. Promoter structure predict...

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Published inZuo wu xue bao Vol. 47; no. 12; p. 2379
Main Authors Wang, Zhen, Zhang, Xiao-Li, Meng, Xiao-Jing, Yao, Meng-Nan, Miu, Wen-Jie, Yuan, Da-Shuang, Zhu, Dong-Ming, Qu, Cun-Min, Lu, Kun, Li, Jia-Na, Liang, Ying
Format Journal Article
LanguageChinese
Published Beijing Chinese Academy of Agricultural Sciences (CAAS) 01.01.2021
Subjects
Apoptosis
Brassica
Brassica napus
Dehydration
Gene expression
Genomes
Hormones
Kinases
MAP kinase
NADH
NADH dehydrogenase
Nicotinamide adenine dinucleotide
Photosynthesis
Plant growth
Protein kinase
Proteins
Quinone oxidoreductase
Quinones
Rape plants
Rapeseed
Stresses
Transcription
Wounding
Yeast
Yeasts
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Summary:Mitogen-activated protein kinases（MAPKs） cascade plays a key role in plant growth and development, division, differentiation, apoptosis, and stress resistance. In this study, a 1612 bp promoter of C group BnMAPK7 gene, designated ProBnMAPK7, was cloned from Brassica napus. Promoter structure prediction by PlantCARE revealed that ProBnMAPK7 contained a lot of ACE, MRE, ABRE, TGACG-motif, and TC-rich repeats cis-acting elements, which involved in light, hormones, defense, and wounding responsiveness. At the same time, we analyzed the expression patterns of MAPK7 genes in Arabidopsis and B. napus, and found that MAPK7 played an important regulatory role in growth and development process and responding to biotic and abiotic stresses. Different lengths of ProBnMAPK7 were gradually ligated to the pCambia1305.1-GUS expression vector to identify the core fragment. GUS histochemical staining analysis showed that the core fragment of ProBnMAPK7 was located in the-467 to-239 bp（ProBnMAPK7-rPE） region. Three copies of th
ISSN:0496-3490